mtnN [2017-8-11 16:0:3]

methylthioadenosine nucleosidase

locus: BSU27270

pI: 4.84

mw: 25.12 kDa

protein length: 231 aa

gene length: 693 bp

function: methionine salvage

product: methylthioadenosine nucleosidase

essential: no

ec: 3.2.2.9

synonyms: yrrU, mtn, mthA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

Gene

Coordinates: 2,787,130 → 2,787,825

The protein

Catalyzed reaction/ biological activity

• S-adenosyl-L-homocysteine + H₂O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine (according to Swiss-Prot)

Protein family

• MtnN subfamily (according to Swiss-Prot)

Structure

• [PDB|1JYS] (from ''Escherichia coli'', 51% identity, 70% similarity) [Pubmed|11591349]

Expression and Regulation

Operons

• genes: [gene|992A24CF9492965BF0E9EEFBB6F0A06871275068|yrrT]-[gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]-[gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA]-[gene|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]-[gene|177EFFDCA6E3C3BB840EED13D9F2D71F99B833B6|yrhC]
  description: [Pubmed|17056751]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|12642660,16885442], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]

Biological materials

Mutant

• MGNA-A850 (yrrU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/850 NBRP B. subtilis, Japan]

References

• 17056751,16513748,12642660,15102328,16885442,24509311